Ruminant DNA detection: Real-time PCR Detection of Ruminant DNA
نویسندگان
چکیده
In order to control the spread of transmissible spongiform encephalopathy (TSE), several DNA methods have been described for the detection of the species origin of meat-andbone-meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semi-quantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element (SINE) Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feedstuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e. heated at 134°C for 3 instead of 20 minutes.
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